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Literature summary for 1.1.1.85 extracted from

  • Miggiano, R.; Martignon, S.; Minassi, A.; Rossi, F.; Rizzi, M.
    Crystal structure of Haemophilus influenzae 3-isopropylmalate dehydrogenase (LeuB) in complex with the inhibitor O-isobutenyl oxalylhydroxamate (2020), Biochem. Biophys. Res. Commun., 524, 996-1002 .
    View publication on PubMed
Search for more literature for 1.1.1.85

Cloned(Commentary)

Cloned (Comment) Organism
expressed in the Escherichia coli BL21 (DE3) Haemophilus influenzae

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the enzyme in complex with the inhibitor O-isobutenyl oxalylhydroxamate and with the cofactor NAD+ at 2.1 A resolution Haemophilus influenzae

Inhibitors

Inhibitors Comment Organism Structure
O-isobutenyl oxalylhydroxamate competitive inhibitor, binds the active site by adopting the same conformation of (2R,3S)-3-isopropylmalate, and to induce an unreported repositioning of the side chain of the amino acids that participate in the coordination of the ligand Haemophilus influenzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+ Haemophilus influenzae the enzyme belongs to the leucine biosynthetic pathway 4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
 ir
(2R,3S)-3-isopropylmalate + NAD+ Haemophilus influenzae ATCC 51907 the enzyme belongs to the leucine biosynthetic pathway 4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
 ir

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae P43860
-
-
Haemophilus influenzae ATCC 51907 P43860
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haemophilus influenzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
 Haemophilus influenzae 4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
 ir
(2R,3S)-3-isopropylmalate + NAD+ the enzyme belongs to the leucine biosynthetic pathway Haemophilus influenzae 4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
 ir
(2R,3S)-3-isopropylmalate + NAD+
-
 Haemophilus influenzae ATCC 51907 4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
 ir
(2R,3S)-3-isopropylmalate + NAD+ the enzyme belongs to the leucine biosynthetic pathway Haemophilus influenzae ATCC 51907 4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
 ir

Synonyms

Synonyms Comment Organism
leuB
-
 Haemophilus influenzae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Haemophilus influenzae

General Information

General Information Comment Organism
drug target since leucine is an essential amino acid for humans, and it is also vital for the growth of many pathogenic bacteria, the enzymes belonging to this pathway can be considered as potential target sites for designing of a new class of antibacterial agents Haemophilus influenzae
metabolism the enzyme belongs to the leucine biosynthetic pathway Haemophilus influenzae